Search results for " VALIDATION"

showing 10 items of 313 documents

Design and experimental validation of a magnetic device for stem cell culture.

2020

Cell culture of bone and tendon tissues requires mechanical stimulation of the cells in order to mimic their physiological state. In the present work, a device has been conceived and developed to generate a controlled magnetic field with a homogeneous gradient in the working space. The design requirement was to maximize the magnetic flux gradient, assuring a minimum magnetizing value in a 15 mm × 15 mm working area, which highly increases the normal operating range of this sort of devices. The objective is to use the machine for two types of biological tests: magnetic irradiation of biological samples and force generation on paramagnetic particles embedded in scaffolds for cell culture. The…

010302 applied physicsElectromagnetic fieldMaterials scienceStem CellsCell Culture TechniquesExperimental validationEquipment Designequipment and supplies01 natural sciencesMagnetic flux010305 fluids & plasmasMagnetic fieldMagnetic FieldsCell cultureDental pulp stem cells0103 physical sciencesMagnetic nanoparticlesIrradiationInstrumentationhuman activitiesBiomedical engineeringThe Review of scientific instruments
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LAI, FAPAR and FCOVER ground-truth map creation from FASat-C satellite imagery and in-situ measurements in Chimbarongo, Chile, for satellite products…

2016

[EN] In remote sensing, validation exercises are essential to ensure the quality of the products originated from satellite Earth observations. To assess the measurement uncertainty derived from satellite products, several ground field data from different ecosystems must be available for use. In the same order of importance, it is necessary to define data sampling and up-scaling methodologies to allow a suitable comparison between the ground data and the pixel size of the product. This paper shows the applied methodology used in the FP7 ImagineS project (Implementing Multi-scale Agricultural Indicators Exploiting Sentinels) to validate 10-days global LAI, FAPAR and vegetation cover products …

010504 meteorology & atmospheric sciencesCampaña de campoGeography Planning and Development0211 other engineering and technologiesFASat-Clcsh:G1-92202 engineering and technology01 natural sciencesBiophysical parametersValidationEarth and Planetary Sciences (miscellaneous)021101 geological & geomatics engineering0105 earth and related environmental sciences2. Zero hungerParámetros biofísicosValidación15. Life on landGeographyField campaign13. Climate actionFASat-C biophysical parameters field campaign validation CopernicusCartographyHumanitieslcsh:Geography (General)CopernicusRevista de Teledetección
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Towards a long-term dataset of ELBARA-II measurements assisting SMOS level-3 land product and algorithm validation at the Valencia Anchor Station

2015

[EN] The Soil Moisture and Ocean Salinity (SMOS) mission was launched on 2nd November 2009 with the objective of providing global estimations of soil moisture and sea salinity. The main activity of the Valencia Anchor Station (VAS) is currently to assist in a long-term validation of SMOS land products. This study focus on a level 3 SMOS data validation with in situ measurements carried out in the period 2010-2012 over the VAS. ELBARA-II radiometer is placed in the VAS area, observing a vineyard field considered as representative of a major proportion of an area of 50×50 km, enough to cover a SMOS footprint. Brightness temperatures (TB) acquired by ELBARA-II have been compared to those obser…

010504 meteorology & atmospheric sciencesMeteorologyGeography Planning and Development0211 other engineering and technologiesData validationlcsh:G1-92202 engineering and technology01 natural sciencesVineyardSoil roughnessFootprintEarth and Planetary Sciences (miscellaneous)Vegetation optical depth14. Life underwaterPrecipitationWater content021101 geological & geomatics engineering0105 earth and related environmental sciencesRadiometerHumedad del suelobrightness temperature ELBARA-II L-MEB SMOS SMOS level 3 data soil moisture soil roughness Valencia Anchor Station vegetation optical depth15. Life on landEspesor óptico de la vegetaciónTerm (time)GeographyL-MEB13. Climate actionBrightness temperatureRugosidad del sueloTemperatura de brilloSoil moistureBrightness temperaturelcsh:Geography (General)
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Technical Note on the quality of DNA sequencing for the molecular characterisation of genetically modified plants

2018

Abstract As part of the risk assessment (RA) requirements for genetically modified (GM) plants, according to Regulation (EU) No 503/2013 and the EFSA guidance on the RA of food and feed from GM plants (EFSA GMO Panel, 2011), applicants need to perform a molecular characterisation of the DNA sequences inserted in the GM plant genome. The European Commission has mandated EFSA to develop a technical note to the applicants on, and checking of, the quality of the methodology, analysis and reporting covering complete sequencing of the insert and flanking regions, insertion site analysis of the GM event, and generational stability and integrity. This Technical Note puts together requirements and r…

0106 biological sciences0301 basic medicineVeterinary (miscellaneous)[SDV]Life Sciences [q-bio]2405 Parasitologymolecularcharacterisationnext‐generation sequencingContext (language use)Plant ScienceComputational biologyGenetically modified cropsBiology01 natural sciencesMicrobiologyGenomeInsert (molecular biology)DNA sequencing03 medical and health sciencessymbols.namesake1110 Plant Sciencegenetically modified organismgenetic stabilityDNA sequencing1106 Food ScienceSanger sequencinggenetically modified organisms2404 Microbiologyrisk assessmentmolecular characterisation10079 Institute of Veterinary Pharmacology and ToxicologyGenetically modified organism3401 Veterinary (miscellaneous)Scientific Opinion030104 developmental biologyNGSsymbols570 Life sciences; biologyAnimal Science and ZoologyParasitologynext-generation sequencing1103 Animal Science and Zoology010606 plant biology & botanyFood ScienceVerification and validation
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Fishery-dependent and -independent data lead to consistent estimations of essential habitats

2016

AbstractSpecies mapping is an essential tool for conservation programmes as it provides clear pictures of the distribution of marine resources. However, in fishery ecology, the amount of objective scientific information is limited and data may not always be directly comparable. Information about the distribution of marine species can be derived from two main sources: fishery-independent data (scientific surveys at sea) and fishery-dependent data (collection and sampling by observers in commercial vessels). The aim of this paper is to compare whether these two different sources produce similar, complementary, or different results. We compare them in the specific context of identifying the Es…

0106 biological scienceshttp://aims.fao.org/aos/agrovoc/c_28840Biodiversité et Ecologiehabitatmodélisation spatialehttp://aims.fao.org/aos/agrovoc/c_38371OceanographyGaleus melastomus01 natural sciencesRessource halieutiquehttp://aims.fao.org/aos/agrovoc/c_38127Scyliorhinus caniculamodèle hiérarchiqueSpatial statisticsEcologymodèle de distributionSampling (statistics)Contrast (statistics)Cross-validationModélisation et simulationGeographyHabitatGestion des pêchesModeling and Simulationhttp://aims.fao.org/aos/agrovoc/c_10566http://aims.fao.org/aos/agrovoc/c_3456http://aims.fao.org/aos/agrovoc/c_38117survey designMarine conservationSpecies Distribution ModelsEcology (disciplines)Bayesian probabilityEtmopterus spinaxenquête statistiqueDonnée sur les pêchesmodèle spatiotemporelSede Central IEOContext (language use)Aquatic ScienceDistribution des populationsBayesian hierarchical models010603 evolutionary biologyhttp://aims.fao.org/aos/agrovoc/c_24026elasmobranchsBiodiversity and Ecologyélasmobrancheétude comparativeBayesian hierarchical models;Cross-validation;Species Distribution Models;Spatial statistics;INLA;elasmobranchs ; survey designINLA14. Life underwaterspecies distribution modelsEcology Evolution Behavior and Systematicshttp://aims.fao.org/aos/agrovoc/c_6113collecte des donnéesÉcologie marinehttp://aims.fao.org/aos/agrovoc/c_29788http://aims.fao.org/aos/agrovoc/c_4609010604 marine biology & hydrobiologyGestion et conservation des pêchescross validation[INFO.INFO-MO]Computer Science [cs]/Modeling and Simulationmodèle bayésienFisheryM01 - Pêche et aquaculture - Considérations généraleshttp://aims.fao.org/aos/agrovoc/c_2a75d27eThéorie bayésienneM40 - Écologie aquatiqueSpatial ecologyhttp://aims.fao.org/aos/agrovoc/c_2942[SDE.BE]Environmental Sciences/Biodiversity and Ecologyvalidation croiséeElasmobranchii
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Dynamic Response of a Rigital Displacement Motor Operating with Various Displacement Strategies

2019

Digital displacement technology has the potential of revolutionizing the performance of hydraulic piston pumps and motors. Instead of connecting each cylinder chamber to high and low pressure in conjunction with the shaft position, two electrically-controlled on/off valves are connected to each chamber. This allows for individual cylinder chamber control. Variable displacement can be achieved by using different displacement strategies, like for example the full stroke, partial stroke, or sequential partial stroke displacement strategy. Each displacement strategy has its transient and steady-state characteristics. This paper provides a detailed simulation analysis of the transient and steady…

0209 industrial biotechnologyExperimental validationControl and OptimizationSteady state (electronics)Digital displacement motorComputer scienceEnergy Engineering and Power Technology02 engineering and technologyVariable displacement01 natural scienceslcsh:Technologylaw.inventionCylinder (engine)PistonTransient response020901 industrial engineering & automationlawControl theoryexperimental validation0103 physical sciencesCylinderDisplacement (orthopedic surgery)Stroke (engine)Transient responsedisplacement strategiesElectrical and Electronic Engineeringsteady-state response010301 acousticsEngineering (miscellaneous)Steady-state responseRenewable Energy Sustainability and the Environmentlcsh:TModelingmodelingDisplacement strategiestransient responseHydraulic cylinderVDP::Teknologi: 500digital displacement motordigital displacement motor; displacement strategies; transient response; steady-state response; modeling; experimental validationTransient (oscillation)Energy (miscellaneous)
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Dry selection and wet evaluation for the rational discovery of new anthelmintics

2017

Helminths infections remain a major problem in medical and public health. In this report, atom-based 2D bilinear indices, a TOMOCOMD-CARDD (QuBiLs-MAS module) molecular descriptor family and linear discriminant analysis (LDA) were used to find models that differentiate among anthelmintic and non-anthelmintic compounds. Two classification models obtained by using non-stochastic and stochastic 2D bilinear indices, classified correctly 86.64% and 84.66%, respectively, in the training set. Equation 1(2) correctly classified 141(135) out of 165 [85.45%(81.82%)] compounds in external validation set. Another LDA models were performed in order to get the most likely mechanism of action of anthelmin…

0301 basic medicineBiophysicsNon-stochastic and stochastic atom-based bilinear indicesBilinear interpolationLDA-based QSAR modelQuBiLs-MAS module01 natural sciencesSet (abstract data type)03 medical and health sciencesMolecular descriptorStatisticsPhysical and Theoretical ChemistryMolecular BiologySelection (genetic algorithm)MathematicsFree and open source softwareTraining setTOMOCOMD-CARDD softwareExternal validationAnthelmintic activityAtom (order theory)Computational creeningCondensed Matter PhysicsLinear discriminant analysis0104 chemical sciencesIndazole010404 medicinal & biomolecular chemistry030104 developmental biologyLead generationMolecular Physics
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First report on the presence of Alloxan in bleached flour by LC-MS/MS method

2017

Abstract In this work the presence of Alloxan in bread, pastry and cake bleached flour was investigate in order to verify possible risk for consumers related to the use of chemicals for flour bleaching. A selective UHPLC–MS/MS method has been developed and validated for the purpose. Alloxan is one of the possible minor side products of oxidation after chemical bleaching of wheat flours, when several chemical agents are used. One hundred and seventy-five flour samples were analyzed for Alloxan determination. The validation of the method was performed in accordance with the ISO/IEC/EN 17025 for linearity, detection limit, quantification limit, accuracy, precision and ruggedness determination.…

0301 basic medicineDetection limitAnalyteChromatographybakery productsSettore CHIM/10 - Chimica Degli AlimentiLC–ESI-MS/MSFlour030209 endocrinology & metabolismFlour Alloxan LC–ESI-MS/MS Validation procedure Bakery productsBiochemistry03 medical and health scienceschemistry.chemical_compound030104 developmental biology0302 clinical medicinechemistryChemical agentsAlloxanLc ms msAlloxanFlour AlloxanBakery productsValidation procedurevalidation procedureFood Science
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Determinants of the Quality of Warfarin Control after Venous Thromboembolism and Validation of the SAMe-TT2-R2 Score: An Analysis of Hokusai-VTE.

2019

Background Time in therapeutic range (TTR) measures the quality of vitamin K antagonist (VKA) anticoagulation. In patients with atrial fibrillation, the dichotomized SAMe-TT2-R2 score (≥2 vs. < 2 points) can predict if adequate TTR is unlikely to be achieved. Aims We validated the SAMe-TT2-R2 score in patients with venous thromboembolism (VTE) randomized to the warfarin arm of the Hokusai-VTE trial. Patients and Methods A total of 3,874 patients were included in the primary analysis (day 31–180 from randomization). The efficacy and safety outcomes were symptomatic recurrent VTE and major or clinically relevant non-major bleeding. Results The rates of recurrent VTE and bleeding events we…

0301 basic medicineMalevitamin K antagonistEXTERNAL VALIDATIONTime FactorsVitamin KWarfarin/therapeutic use030204 cardiovascular system & hematologyTHERAPYSeverity of Illness Indexlaw.inventionchemistry.chemical_compound0302 clinical medicineRandomized controlled trialEdoxabanlawRecurrenceAtrial FibrillationVitamin K/antagonists & inhibitorsStrokeRISKAtrial fibrillationHematologyVenous ThromboembolismVitamin K antagonistMiddle Agedrisk assessment modelTIMEPREDICTSTreatment OutcomeAnticoagulants/therapeutic useResearch DesignANTICOAGULATION CONTROLFemaleLife Sciences & Biomedicinemedicine.drugHemorrhage/drug therapyAdultmedicine.medical_specialtyRandomizationmedicine.drug_classvenous thromboembolismHemorrhageRisk AssessmentSensitivity and SpecificityEDOXABAN03 medical and health sciencesDouble-Blind MethodVITAMIN-K ANTAGONISTSInternal medicinemedicineNONVALVULAR ATRIAL-FIBRILLATIONORAL ANTICOAGULANTHumansInternational Normalized RatioBlood CoagulationScience & Technologybusiness.industryquality of treatmentWarfarinAnticoagulantsmedicine.diseasewarfarinClinical trial030104 developmental biologyPeripheral Vascular DiseasechemistryBlood Coagulation/drug effectsAtrial Fibrillation/bloodCardiovascular System & CardiologyLinear ModelsWarfarinbusinessVenous Thromboembolism/drug therapyThrombosis and haemostasis
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A multicentric study to evaluate the use of relative retention times in targeted proteomics.

2016

Despite the maturity reached by targeted proteomic strategies, reliable and standardized protocols are urgently needed to enhance reproducibility among different laboratories and analytical platforms, facilitating a more widespread use in biomedical research. To achieve this goal, the use of dimensionless relative retention times (iRT), defined on the basis of peptide standard retention times (RT), has lately emerged as a powerful tool. The robustness, reproducibility and utility of this strategy were examined for the first time in a multicentric setting, involving 28 laboratories that included 24 of the Spanish network of proteomics laboratories (ProteoRed-ISCIII). According to the results…

0301 basic medicineMultiple reaction monitoringProteomicsBiomedical ResearchComputer scienceBiophysicsLiquid chromatographyContext (language use)BioinformaticsBiochemistry03 medical and health sciencesInter-laboratory validationTargeted proteomicsObserver VariationReproducibilityResearchReproducibility of ResultsAnalytical scienceReference StandardsStandardizationReproducibilityCell and molecular biologyTargeted proteomics030104 developmental biologyBiological significanceBiochemical engineeringRetention timeChromatography LiquidJournal of proteomics
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